Publications

2025

Functional Enrichment and Sequence-Based Discovery Identify Promiscuous and Efficient Poly Lactic Acid Degrading Enzymes

G. Stojanovski, M. Bawn, A. Locks, E. Ambrose-Dempster, J. M. Ward,
J. W. E. Jeffries, and H. C. Hailes, 2025, Environ. Sci. Technol. 59, 8602−8613. DOI: 10.1098/rsos.241270.

Comparison of the behaviour of pro-oxidant additive containing plastic degradation in the unmanaged natural environment and in the laboratory

F. Sciscione, L. Prout, J. W. E. Jeffries, H. J. Karam, A. Constantinou, F. Peng, S. M. Al-Salem, H. C. Hailes, and M. Miodownik, 2025, R. Soc. Open Sci. 12(3). DOI: 10.1098/rsos.241270.

3D Printing with tuneable degradation: Thiol-ene and thiol-yne containing formulations for biomedical applications

A. Locks, B.J. Bowles, S. Brown, H.C. Hailes, 2025, International Journal of Pharmaceutics, 674, 125432. DOI: 10.1016/j.ijpharm.2025.125432.

Modulation-enabled healable and stretchable shape-memory polymer composites for digital light processing 4D printing

W. Huanga, W. Chen, V. Singh, J. Zhanga, Y. Wang, M. Alabdullatif, E. Bele, G. J. Lye, H. C. Hailes, M. K. Tiwari, 2025, Additive Manufacturing, 101, 104699. DOI: 10.1016/j.addma.2025.104699.

Using hyperspectral imaging and machine learning to identify food-contaminated compostable and recyclable plastics

N. Taneepanichskul, H.C. Hailes, M. Miodownik, 2025, UCL Open: Environment, 7(4). DOI: 10.14324/111.444/ucloe.3237.

2024

Broadening The Substrate Scope of Aldolases Through Metagenomic Enzyme Discovery

A. Rizzo, C. Aranda, J. Galman, A. Alcasabas, A. Pandya, A. Bornadel, B. Costa, H. C. Hailes, J. M. Ward, J. W. E. Jeffries, and B. Dominguez, 2024, ChemBioChem, e202400278.

BioLindlar Catalyst: Ene-Reductase-Promoted Selective Bioreduction of Cyanoalkynes to Give (Z)-Cyanoalkenes

J. González-Rodríguez, S. González-Granda, H. Kumar, O. Alvizo, L. Escot, H. C. Hailes, V. Gotor-Fernández, and I. Lavandera, 2024, Angew. Chem. Int. Ed., 63, e202410283.

β,β-Disubstituted Alkan-2-ones from Propargylic Alcohols Combining a Meyer-Schuster Rearrangement and Asymmetric Alkene Bioreduction

L. Escot, S. Gonzalez-Granda, D. Méndez-Sánchez, Y. Wang, H. Hailes, I. Lavandera, and V. Gotor-Fernández, 2024, Adv. Synth. Catal. DOI: 10.1002/adsc.202400653.

A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines

Y. Wang, Y. Li, Y. Ni, D.-K. Bučar, P. A. Dalby, J. M. Ward, J. W. E. Jeffries, H. C. Hailes, 2024, Catal. Sci. Technol., 14, 2390–2399.

A one-step method for generating antimicrobial nanofibre meshes via coaxial electrospinning

F. Zhang, A. I. Jacobs, M. Woodall, H. C. Hailes, I. F. Uchegbu, D. Fernandez-Reyes, C. M. Smith, K. Dziemidowicz, G. R. Williams, 2024, Mater. Adv. 5, 5561–5571.

Using hyperspectral imaging to identify and classify large microplastic contamination in industrial composting processes

N. Taneepanichskul, H. C. Hailes, M. Miodownik, Front. Sustain., 2024, 5, 1332163.

Natural transaminase fusions for biocatalysis

L. Prout, H. C. Hailes, J. M. Ward, RSC Advances, 2024, 14, 4264.

An Alternative Cascade for the Selective Methylation of Catechols and Tetrahydroisoquinolines by O-Methyltransferases

M. T. Salinger, D. Castellano Garrido, E. D. Lamming, J. M. Ward, T. S. Moody, J. W. E. Jeffries, H. C. Hailes, ChemCatChem, 2024, e202400492.

2023

Expanding the Substrate Scope of N– and O– Methyltransferases from Plants for Chemoselective Alkylation

E. Jockmann, F. Subrizi, M. K. F. Mohr, E. M. Carter, P. M. Hebecker, D. Popadić, H. C. Hailes, J. N. Andexer, ChemCatChem, 2023, 15, e202300930.

Automatic identification and classification of compostable and biodegradable plastics using hyperspectral imaging

N. Taneepanichskul, H. C. Hailes, M. Miodownik, Frontiers in Sustainability, 2023, 4, 1125954.

C-1 Substituted isoquinolines potentiate the antimycobacterial activity of rifampicin and ethambutol

L.T. Martin, E. D. Lamming, A. Maitra, P. N. Mortazavi, R. Roddan, J. M. Ward, S. Bhakta, H. C. Hailes, Frontiers in Antibiotics, 2023, 2, 1095013.

Mechanoenzymatic reactions for the hydrolysis of PET

E. Ambrose-Dempster, L. Leipold, D. Dobrijevic, M. Bawn, E. M. Carter, G. Stojanovski, T. D. Sheppard, J. W. E. Jeffries, J. M. Ward, H. C. Hailes, RSC Advances, 2023, 13, 9954-9962.

Multi-enzyme catalysed processes using purified and whole-cell biocatalysts towards a 1,3,4-substituted tetrahydroisoquinoline

D. Weber, L. de Souza Bastos, M. Winkler, Y. Ni, A. E. Aliev, H. C. Hailes, D. Rother, RSC Advances, 2023, 13, 10097-10109.

The Discovery of Imine Reductases and their Utilisation for the Synthesis of Tetrahydroisoquinolines

M. Cárdenas‐Fernández, R. Roddan, E. M. Carter, H. C. Hailes, J. M. Ward, ChemCatChem, 2023.

The performance and environmental impact of pro-oxidant additive containing plastics in the open unmanaged environment—a review of the evidence

F. Sciscione F, H.C. Hailes, M. Miodownik, Royal Society Open Science, 2023, 10, 230089.

The use of tyrosinases in a chemoenzymatic cascade as a peptide ligation strategy

Y. Ni, Y. Wang, A. B. Tabor, J. M. Ward, H. C. Hailes, RSC Chemical Biology, 2023.

Understanding and optimising the transfection of lipopolyplexes formulated in saline: the effects of peptide and serum

L. Cui, L. Kudsiova, F. Campbell, D. J. Barlow, H. C. Hailes, A. B. Tabor, M. J. Lawrence, Biomaterials Science, 2023.

2022

Chemoenzymatic approaches to plant natural product inspired compounds

R. Roddan, E.M. Carter, B. Thair, H.C. Hailes, Natural Product Reports, 2022, 39, 1375-1382.

Methyltransferases: Functions and Applications

E. Abdelraheem, B. Thair, R.F. Varela, E. Jockmann, D. Popadic, H.C. Hailes, J.M. Ward, A.M. Iribarren, E.S. Lewkowicz, J.N. Andexer, P.L. Hagedoorn, U. Hanefeld, ChemBioChem, 2022, e202200212.

Enzymatic synthesis of benzylisoquinoline alkaloids using a parallel cascade strategy and tyrosinase variants

Y. Wang, F. Subrizi, E.M. Carter, T.D. Sheppard, J.M. Ward, H.C. Hailes, Nat. Commun., 2022, 13, 5436.

Mechanoenzymatic reactions with whole cell transaminases: shaken, not stirred

E.M. Carter, E. Ambrose-Dempster, J.M. Ward, T.D. Sheppard, H.C. Hailes, Green Chem., 2022, 24, 3662-6.

Selective Transaminase-Mediated Synthesis of Serotonin and Melatonin Receptor Agonists

D. Baud, N. Tappertzhofen, T.S. Moody, J.M. Ward, H.C. Hailes, Adv. Synth. Catal., 2022, 364, 1564-72.

Chemoenzymatic approaches to plant natural product inspired compounds

R. Roddan, E.M. Carter, B. Thair, H.C. Hailes, Nat. Prod. Rep., 2022, online publication

Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore

O. Tau, A. Henley, A.N. Boichenko, N.N. Kelshchine, R. Riley, B. Wang, D. Winning, R. Lewin, I.P. Parkin, J.M. Ward, H.C. Hailes, A.V. Bochenkova, H.H. Fielding, Nat. Commun., 2022, 13, 507.

2021

A photolectron imaging study of the deprotonated GFP chromophore anion and RNA fluorescent tags

J.L. Woodhouse, A. Henley, R. Lewin, J.M. Ward, H.C. Hailes, A.V. Bochenkova, H.H. Fielding, Phys. Chem. Chem. Phys. 2021, 23, 19911.

Chemoenzymatic Cascades toward Methylated Tetrahydroprotoberberine and Protoberberine Alkaloids

R. Roddan, F. Subrizi, J. Broomfield, J.M. Ward, N.H. Keep, H.C. Hailes, Org. Lett., 2021, 16, 6342-7.

Characterisation of a hyperthermophilic transketolase from Thermotoga maritima DSM3109 as a biocatalyst for 7-keto-octuronic acid synthesis

M. Cárdenas-Fernández, F. Subrizi, D. Dobrijevic, H.C. Hailes, J.M. Ward, Org. Biomol. Chem., 2021, 19, 6493.

Direct Conversion of Hydrazones to Amines using Transaminases

E. M. Carter, F. Subrizi, J.M. Ward, T.D. Sheppard, H.C. Hailes, ChemCatChem, available online ahead of publication.

The impact and effectiveness of the general public wearing masks to reduce the spread of pandemics in the UK: a multidisciplinary comparison of single-use masks versus reusable face masks

A.L Allison, E. Ambrose-Dempster, M. Bawn, M. Casas Arrendondo, C. Chau, K. Chandler, D. Dobrijevic, T. Domenech Aparasi, H.C. Hailes, P. Lettieri, C. Liu, F. Medda, S. Michie, M. Miodownik, B. Munro, D. Purkiss, J.M., UCL Open: Environment, 2021, 3:01

Multienzyme One-Pot Cascades Incorporating Methyltransferases for the Strategic Diversification of Tetrahydroisoquinoline Alkaloids

F. Subrizi, Y. Wang, B. Thair, D. Mendez-Sanchez, R. Roddan, M. Cardenas-Fernandez, J. Siegrist, M. Richter, J.N. Andexer, J.M. Ward, H.C. Hailes, Angew. Chemie – Int. Ed., 2021, 60, 18673-9.

Discovery of New Carbonyl Reductases Using Functional Metagenomics and Applications in Biocatalysis

S.A. Newgas, J.W.E. Jeffries, T.S. Moody, J.M. Ward, H.C. Hailes, Adv. Synth. Catal., 2021, 363, 3044-52.

A bicyclic S-adenosylmethionine regeneration system applicable with different nucleosides or nucleotides as cofactor building blocks

D. Popadić, D. Mhaindarkar, M.H.N. Dang Thai, H.C. Hailes, S. Mordhorst, J.N. Andexer, RSC Chem. Biol. 2021, 2, 883-91.

Regioselective Dehydration of Sugar Thioacetals under Mild Conditions

R. Szpara, A. Goyder, M.J. Porter, H.C. Hailes, T.D. Sheppard, Org. Lett. 2021, 23, 2488-92.

Norcoclaurine Synthase-Mediated Stereoselective Synthesis of 1,1′-Disubstituted, Spiro- And Bis-Tetrahydroisoquinoline Alkaloids

J. Zhao, D. Méndez-Sánchez, R. Roddan, J.M. Ward, H.C. Hailes, ACS Catal., 2021, 11, 131-8.

2020

Single step syntheses of (1S)-aryl-tetrahydroisoquinolines by norcoclaurine synthases

R. Roddan, A. Sula, D. Méndez-Sánchez, F. Subrizi, B.R. Lichman, J. Broomfield, M. Richter, J.N. Andexer, J.M. Ward, N.H. Keep, H.C. Hailes, Commun. Chem. 2020 3, 170.

pET expression vector customized for efficient seamless cloning

D. Dobrijevic, L.A. Nematollahi, H.C. Hailes, J.M. Ward, BioTechniques, 2020, 69, 384-7.

Carprofen elicits pleiotropic mechanisms of bactericidal action with the potential to reverse antimicrobial drug resistance in tuberculosis

A. Maitra, D. Evangelopoulos, A. Chrzastek, L.T. Martin, A. Hanrath, E. Chapman, H.C. Hailes, M. Lipman, T.D. McHugh, S.J. Waddell, S. Bhakta, J. Antimicrob. Chemother., 2020, 75, 3194-201.

Identification and catalytic properties of new epoxide hydrolases from the genomic data of soil bacteria

G. Stojanovski, D. Dobrijevic, H.C. Hailes, J.M. Ward, Enzyme Microb. Technol., 2020, 139, 109592.

Multi-modal imaging probe for assessing the efficiency of stem cell delivery to orthotopic breast tumours

M. Zaw Thin, H. Allan, R. Bofinger, T.D. Kostelec, S. Guillaume, J.J. Connell, P.S. Patrick, H.C. Hailes, A.B. Tabor, M.F. Lythgoe, D.J. Stuckey, T.L. Kalber, Nanoscale, 2020, 12, 16570-85.

Characterisation of four hotdog-fold thioesterases for their implementation in a novel organic acid production system

T.W.P. Hickman, D. Baud, L. Benhamou, H.C. Hailes, J.M. Ward, App. Microbiol. Biotechnol. 2020, 104, 4397-406.

Engineering transketolase to accept both unnatural donor and acceptor substrates and produce α-hydroxyketones

H. Yu, R.I. Hernandez Lopez, D. Steadman, D. Méndez-Sánchez, S. Higson, A. Cazares-Korner, T.D. Sheppard, J.M. Ward, H.C. Hailes, P.A. Dalby, FEBS J., 2020, 287, 1758-76.

Pictet–Spenglerases in alkaloid biosynthesis: Future applications in biocatalysis

R. Roddan, J.M. Ward, N.H. Keep, H.C. Hailes, Curr. Opp. Chem. Biol., 2020, 55, 69-75.

2019

Acceptance and Kinetic Resolution of α-Methyl-Substituted Aldehydes by Norcoclaurine Synthases

R. Roddan, G. Gygli, D. Méndez-Sánchez, J. Pleiss, J.M. Ward, N.H. Keep, H.C. Hailes, ACS Catal., 2019, 9, 9640-9.

Effect of Liposomal Encapsulation on the Chemical Exchange Properties of Diamagnetic CEST Agents

E. Demetriou, H.E. Story, R. Bofinger, H.C. Hailes, A.B. Tabor, X. Golay, J. Phys. Chem. B, 2019, 123, 7545-57.

Design and Use of de novo Cascades for the Biosynthesis of New Benzylisoquinoline Alkaloids

Y. Wang, N. Tappertzhofen, D. Méndez-Sánchez, M. Bawn, B. Lyu, J.M. Ward, H.C. Hailes, Angew. Chemie – Int. Ed., 2019, 58, 10120-5.

Biomimetic Phosphate-Catalyzed Pictet-Spengler Reaction for the Synthesis of 1,1′-Disubstituted and Spiro-Tetrahydroisoquinoline Alkaloids

J. Zhao, D. Méndez-Sánchez, J.M. Ward, H.C. Hailes, J. Org. Chem., 2019, 84, 7702-10.

Aminopolyols from Carbohydrates: Amination of Sugars and Sugar-Derived Tetrahydrofurans with Transaminases

F. Subrizi, L. Benhamou, J.M. Ward, T.D. Sheppard, H.C. Hailes, Angew. Chemie – Int. Ed., 2019, 58, 3854-8.

Metagenomic ene-reductases for the bioreduction of sterically challenging enones

D. Dobrijevic, L. Benhamou, A.E. Aliev, D. Méndez-Sánchez, N. Dawson, D. Baud, N. Tappertzhofen, T.S. Moody, C.A. Orengo, H.C. Hailes, J.M. Ward, RSC Adv., 2019, 63, 36608-14.

Catalytic direct amidations in tert-butyl acetate using B(OCH₂CF₃)₃

C.E. Coomber, V. Laserna, L.T. Martin, P.D. Smith, H.C. Hailes, M.J. Porter, T.D. Sheppard, Org. Biomol. Chem., 2019, 17, 6465-9.

Functionalised tetrahydrofuran fragments from carbohydrates or sugar beet pulp biomass

L. Benhamou, R.W. Foster, D.P. Ward, K. Wheelhouse, L. Sloan, C.J. Tame, D.-K. Bucar, G.J.Lye, H.C. Hailes, T.D. Sheppard, Green Chem., 21, 2035-42.

The discovery and enhanced properties of trichain lipids in lipopolyplex gene delivery systems

A. Mohammadi, L. Kudsiova, M.F.M. Mustapa, F. Campbell, D. Vlaho, K. Welser, H. Story, A.D. Tagalakis, S.L. Hart, D.J. Barlow, A.B. Tabor, M.J. Lawrence, H.C. Hailes, Org. Biomol. Chem., 2019, 17, 945-57.

The identification and use of robust transaminases from a domestic drain metagenome

L. Leipold, D. Dobrijevic, J.W.E. Jeffries, M. Bawn, T.S. Moody, J.M. Ward, H.C. Hailes, Green Chem., 21, 75-86.

Trichain cationic lipids: The potential of their lipoplexes for gene delivery

L. Kudsiova, A Mohammadi, M.F.M. Mustapa, F. Campbell, K. Welser, D. Vlaho, H. Story, D.J. Barlow, A.B. Tabor, H.C. Hailes, M.J. Lawrence, Biomat. Sci., 7, 149-58.

2018

Development of lipopolyplexes for gene delivery: A comparison of the effects of differing modes of targeting peptide display on the structure and transfection activities of lipopolyplexes

R. Bofinger, M. Zaw-Thin, N.J. Mitchell, P.S. Patrick, C. Stowe, A. Gomez-Ramirez, H.C. Hailes, T.L. Kalber, A.B. Tabor, J. Pept. Sci., 24, e3131.

One-pot, two-step transaminase and transketolase synthesis of L-gluco-heptulose from L-arabinose

M. Bawn, F. Subrizi, G.J. Lye, T.D. Sheppard, H.C. Hailes, J.M. Ward, Enzyme Microb. Technol., 116, 16-22.

Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from L-arabinose

M. Bawn, F. Subrizi, G.J. Lye, T.D. Sheppard, H.C. Hailes, J.M. Ward, Data Brief, 19, 1341-54.

Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations

H. Lechner, P. Soriano, R. Poschner, H.C. Hailes, J.M. Ward, W. Kroutil, Biotech. J., 13, 1700542.

Enzymatic synthesis of chiral amino-alcohols by coupling transketolase and transaminase-catalyzed reactions in a cascading continuous-flow microreactor system

P. Gruber, F. Carvalho, M.P.C. Marques, B. O’Sullivan, F. Subrizi, D. Dobrijevic, J. Ward, H.C. Hailes, P. Fernandes, R. Wohlgemuth, F. Baganz, N. Szita, Biotech. Bioeng., 2018, 115, 586-96. 

Development of a microwave-assisted sustainable conversion of furfural hydrazones to functionalised phthalimides in ionic liquids

V. Karaluka, K. Murata, S. Masuda, Y. Shiramatsu, T. Kawamoto, H.C. Hailes, T.D. Sheppard, A. Kamimura, RSC Adv., 2018, 8, 22617-24.

One-pot chemoenzymatic synthesis of trolline and tetrahydroisoquinoline analogues

J. Zhao, B.R. Lichman, J.M. Ward, H.C. Hailes, Chem. Commun., 2018, 54, 1323-6.

2017

Structural Evidence for the Dopamine-First Mechanism of Norcoclaurine Synthase

B.R. Lichman, A. Sula, T. Pesnot, H.C. Hailes, J.M. Ward, N.H. Keep, Biochem., 2017, 56, 5274-7.

Enzymatic and Chemoenzymatic Three-Step Cascades for the Synthesis of Stereochemically Complementary Trisubstituted Tetrahydroisoquinolines

V. Erdmann, B.R. Lichman, J. Zhao, R.C. Simon, W. Kroutil, J.M. Ward, H.C. Hailes, D. Rother, Angew. Chemie – Int. Ed., 56, 12503-7.

Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor

G. Weitsman, N.J. Mitchell, R. Evans, A. Cheung, T.L. Kalber, R. Bofinger, G.O. Fruhwirth, M. Keppler, Z.V.F. Wright, P.R. Barber, P. Gordon, T. De Koning, W. Wulaningsih, K. Sander, B. Vojnovic, S. Ameer-Beg, M. Lythgoe, J.N. Arnold, E. Arstad, F. Festy, H.C. Hailes, A.B. Tabor, Oncogene, 36, 3618-28.

Tunable Semiconducting Polymer Nanoparticles with INDT-Based Conjugated Polymers for Photoacoustic Molecular Imaging

T. Stahl, R. Bofinger, I. Lam, K.J. Fallon, P. Johnson, O. Ogunlade, V. Vassileva, R.B. Pedley, P.C. Beard, H.C. Hailes, H. Bronstein, A.B. Tabor, Bioconj. Chem., 28, 1734-40.

Preparation of 5-hydroxymethylfurfural from glucose and fructose in ionic liquids by reactive vacuum distillation over a solid catalyst

M.B. Nasirudeen, H.C. Hailes, J.R.G. Evans, Curr. Org. Synth., 14, 596-603.

Enzyme catalysed Pictet-Spengler formation of chiral 1,1′-disubstituted- and spiro-tetrahydroisoquinolines

B.R. Lichman, J. Zhao, H.C. Hailes, J.M. Ward, Nat. Commun., 8, 14883.

One-pot phosphate-mediated synthesis of novel 1,3,5-trisubstituted pyridinium salts: A new family of S. aureus inhibitors

T. Pesnot, M.C. Gershater, M. Edwards, J.M. Ward, H.C. Hailes, Molecules, 22, 626.

The Effect of Conjugation on the Competition between Internal Conversion and Electron Detachment: A Comparison between Green Fluorescent and Red Kaede Protein Chromophores

J. Tay, M.A. Parkes, K. Addison, Y. Chan, L. Zhang, H.C. Hailes, P.C. Bulman Page, S.R. Meech, L. Blancafort, H.H. Fielding, J. Phys. Chem. Lett., 8, 765-71.

An integrated biorefinery concept for conversion of sugar beet pulp into value-added chemicals and pharmaceutical intermediates

M. Cardenas-Fernandez, M. Bawn, C. Hamley-Bennett, P.K.V. Bharat, F. Subrizi, N. Suhaili, D.P. Ward, S. Bourdin, P.A. Dalby, H.C. Hailes, P. Hewitson, S. Ignatova, Faraday Discuss., 202, 415-31.

A metagenomics approach for new biocatalyst discovery: Application to transaminases and the synthesis of allylic amines

D. Baud, J.W.E. Jeffries, T.S. Moody, J.M. Ward, H.C. Hailes, Green Chem., 19, 1134-43.

Mechanism of resonant electron emission from the deprotonated GFP chromophore and its biomimetics

A.V. Bochenkova, C.R.S. Mooney, M.A. Parkes, J.L. Woodhouse, L. Zhang, R. Lewin, J.M. Ward, H.C. Hailes, L.H. Andersen, H.H. Fielding, Chem. Sci., 8, 3154-63.

Ortho and para chromophores of green fluorescent protein: controlling electron emission and internal conversion

C. McLaughlin, M. Assmann, M.A. Parkes, J.L. Woodhouse, R. Lewis, H.C. Hailes, G.A. Worth, H.H. Fielding, Chem. Sci.

Furfurylamines from biomass: Transaminase catalysed upgrading of furfurals

A. Dunbabin, F. Subrizi, J.M. Ward, T.D. Sheppard, H.C. Hailes, Green. Chem., 19, 397-404.

2016

Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes

P.E. Affaticati, S.-B. Dai, P. Payongsri, H.C. Hailes, K. Tittmann, P.A. Dalby, Sci. Rep., 6, 35716.

Strategies for synthesis of epoxy resins from oleic acid derived from food wastes

T. Hayes, Y. Hu, S.A. Sanchez-Vazquez, H.C. Hailes, A.E. Aliev, J.R.G. Evans, J. Polym. Sci.

Structure-Function Studies on the Chemo- and Stereoselectivity of ThDP-Dependent Enzymes

A. Baierl, C. Vogel, J. Pleiss, H.C. Hailes, M. Muller, M. Pohl, Chem. Ing. Tech., 88, 1247.

Delivery of siRNA using ternary complexes containing branched cationic peptides: The role of peptide sequence, branching and targeting

L. Kudsiova, K. Welser, F. Campbell, A. Mohammadi, N. Dawson, L. Cui, H.C. Hailes, M.J. Lawrence, A.B. Tabor, Mol. Biosyst., 12, 934-51.

Transketolase catalysed upgrading of L-arabinose: The one-step stereoselective synthesis of L-gluco-heptulose

F. Subrizi, M. Cárdenas-Fernández, G.J. Lye, J.M. Ward, P.A. Dalby, T.D. Sheppard, H.C. Hailes, Green. Chem., 18, 3158-65.

Chemical cascades in water for the synthesis of functionalized aromatics from furfurals

S. Higson, F. Subrizi, T.D. Sheppard, H.C. Hailes, Green Chem., 18, 1855-8.

2015

Multi-step biocatalytic strategies for chiral amino alcohol synthesis

M.F. Villegas-Torres, R.J. Martinez-Torres, A. Cazeres, H.C. Hailes, F. Baganz, J. Ward, Enzyme Microb. Technol., 81, 23-30.

Sustainable Synthesis of Chiral Tetrahydrofurans through the Selective Dehydration of Pentoses

R.W. Foster, C.J. Tame, D.-K. Bučar, H.C. Hailes, T.D. Sheppard, Chem. Eur. J., 21, 15947-50.

A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase

D. Baud, N. Ladkau, T.S. Moody, J.M. Ward, H.C. Hailes, Chem. Commun., 51, 17225-8.

ω-Transaminases for the amination of functionalised cyclic ketones

N. Richter, R.C. Simon, H. Lechner, W. Kroutil, J.M. Ward, H.C. Hailes, Org. Biomol. Chem., 13, 8843-51. 

Single active-site mutants are sufficient to enhance serine:pyruvate α-transaminase activity in an ω-transaminase

D. Deszcz, P. Affaticati, N. Ladkau, A. Gelel, J.M. Ward, H.C. Hailes, P.A. Dalby, FEBS J., 13, 2512-26.

Irreversible endo-Selective Diels-Alder Reactions of Substituted Alkoxyfurans: A General Synthesis of endo-Cantharimides

R.W. Foster, L. Benhamou, M.J. Porter, D.-K. Bucar, H.C. Hailes, C.J. Tame, T.D. Sheppard, Chem. Eur. J., 21, 6107-14.

Second generation engineering of transketolase for polar aromatic aldehyde substrates

P. Payongsri, D. Steadman, H.C. Hailes, P.A. Dalby, Enzyme Microb. Technol., 71, 45-52.

One-pot triangular chemoenzymatic cascades for the syntheses of chiral alkaloids from dopamine

B.R. Lichman, E.D. Lamming, T. Pesnot, J.M. Smith, H.C. Hailes, J.M. Ward, Green Chem., 17, 852-5.

Modelling and optimisation of the one-pot, multi-enzymatic synthesis of chiral amino-alcohols based on microscale kinetic parameter determination

L. Rios-Solis, P. Morris, C. Grant, A.O.O. Odeleye, H.C. Hailes, J.M. Ward, P.A. Dalby, F. Baganz, G.J. Lye, Chem. Eng. Sci., 122, 360-72.

Dopamine-first mechanism enables the rational engineering of the norcoclaurine synthase aldehyde activity profile

B.R. Lichman, M.C. Gershater, E.D. Lamming, T. Pesnot, A. Sula, N.H. Keep, H.C. Hailes, J.M. Ward, FEBS J., 282, 1137-51.

2014

Tetrahydroisoquinolines affect the whole-cell phenotype of Mycobacterium tuberculosis by inhibiting the ATP-dependent MurE ligase

J.D. Guzman, T. Pesnot, D.A. Barrera, H.M. Davies, E. McMahon, D. Evangelopoulos, P.N. Mortazavi, T. Munshi, A. Maitra, E.D. Lamming, R. Angell, M.C. Gershater, J. Antimicrob. Chemother., 70, 1691-703.

Competition between photodetachment and autodetachment of the 2¹ ππ* state of the green fluorescent protein chromophore anion

C.R.S. Mooney, M.A. Parkes, L. Zhang, H.C. Hailes, A. Simperler, M.J. Bearpark, H.H. Fielding, J. Chem. Phys., 140, 205103.

Synthesis of pharmaceutically relevant 17-α-amino steroids using an ω-transaminase

N. Richeter, R.C. Simon, W. Kroutil, J.M. Ward, H.C. Hailes, Chem. Commun., 50, 6098-100.

Gold catalysed synthesis of 3-alkoxyfurans at room temperature

M.N. Pennell, R.W. Foster, P.G. Turner, H.C. Hailes, C.J. Tame, T.D. Sheppard, Chem. Commun., 50, 1302-4.

The selective conversion of D-limonene to p,α-dimethylstyrene

S.A. Sanchez-Vazquez, T.D. Sheppard, J.R.G. Evans, H.C. Hailes, RSC Adv., 4, 61652-5.

Long-term stabilization of reflective foams in sea water

A. Aziz, H.C. Hailes, J.M. Ward, J.R.G. Evans, RSC. Adv., 4, 53028-36.

Resonantly enhanced multiphoton ionization spectrum of the neutral green fluorescent protein chromophore

J.B. Greenwood, J.Miles, S.D.Camillis, P. Mulholland, L. Zhang, M.A. Parkes, H.C. Hailes, H.H. Fielding, J. Phys. Chem. Lett., 5, 3588-92.

Efficient 2-step biocatalytic strategies for the synthesis of all nor(pseudo)ephedrine isomers

T. Sehl, H.C. Hailes, J.M. Ward, U. Menyes, M. Pohl, D. Rother, Green Chem., 16, 3341-8.

The substrate specificity, enantioselectivity and structure of the (R)-selective amine: Pyruvate transaminase from Nectria haematococca

C. Sayer, R.J. Martinez-Torres, N. Richter, M.N. Isupov, H.C. Hailes, J.A. Littlechild, J.M. Ward, FEBS J., 281, 2240-53.

2013

Engineering stereoselectivity of ThDP-dependent enzymes

H.C. Hailes, D. Rother, M. Muller, R. Westphal, J.M. Ward, J. Pleiss, C. Vogel, M. Pohl, FEBS J., 280, 6374-94.

Multifunctional receptor-targeted nanocomplexes for the delivery of therapeutic nucleic acids to the Brain

G.D. Kenny, A.S. Bienemann, A.D. Tagalakis, J.A.Pugh, K. Welser, F. Campbell, A.B. Tabor, H.C. Hailes, S.S. Gill, M.F. Lythgoe, C.W. McLeod, E.A. White, Biomaterials, 36, 9190-200.

Hydrophobic polymers from food waste: Resources and synthesis

S.A. Sanchez-Vazquez, H.C. Hailes, J.R.G. Evans, Polym. Rev., 53, 627-94.

Highly regioselective synthesis of substituted isoindolinones via ruthenium-catalyzed alkyne cyclotrimerizations

R.W. Foster, C.J. Tame, H.C. Hailes, T.D. Sheppard, Adv. Synth. Catal., 355, 2353-60.

Two steps in one pot: Enzyme cascade for the synthesis of nor(pseudo)ephedrine from inexpensive starting materials

T. Sehl, H.C. Hailes, J.M. Ward, R. Wardenga, E. Von Lieres, H. Offermann, R. Westphal, M. Pohl, D. Rother, Angew. Chemie – Int. Ed., 52, 6772-5.

Gene delivery using ternary lipopolyplexes incorporating branched cationic peptides: The role of peptide sequence and branching

K. Welser, F. Campbell, L. Kudsiova, A. Mohammadi, N. Dawson, S.L. Hart, D.J. Barlow, H.C. Hailes, M.J. Lawrence, A.B. Tabor, Mol. Pharm., 10, 127-41.

Incorporation of paramagnetic, fluorescent and PET/SPECT contrast agents into liposomes for multimodal imaging

N. Mitchell, T.L. Kalber, M.S. Cooper, K. Sunassee, S.L. Chalker, K.P. Shaw, K.L. Ordidge, A. Badar, S.M. Janes, P.J. Blower, M.F. Lythgoe, H.C. Hailes, Biomaterials, 34, 1179-92.

2012

Rational substrate and enzyme engineering of transketolase for aromatics

P. Payongsri, D. Steadman, J. Strafford, A. MacMurray, H.C. Hailes, P.A. Dalby, Org. Biomol. Chem., 10, 9021-9.

The catalytic potential of Coptis japonica NCS2 revealed – Development and utilisation of a fluorescamine-based assay ETI

T. Pesnot, M.C. Gershater, J.M. Ward, H.C. Hailes, Adv. Synth. Catal., 345, 2997-3008.

Multifunctional receptor-targeted nanocomplexes for magnetic resonance imaging and transfection of tumours

G.D. Kenny, C. Villegas-Llerena, A.D. Tagalakis, F. Campbell, K. Welser, M. Botta, A.B. Tabor, H.C. Hailes, M.F. Lythgoe, S.L. Hart, Biomaterials, 33, 7241-50.

TTC-based screening assay for ω-transaminases: A rapid method to detect reduction of 2-hydroxy ketones

T. Sehl, R.C. Simon, H.C. Hailes, J.M. Ward, U. Schell, M. Pohl, D. Rother, J. Biotechnol., 159, 188-94.

Modular microfluidic reactor and inline filtration system for the biocatalytic synthesis of chiral metabolites

B. O’Sullivan, H. Al-Bahrani, J. Lawrence, M. Campos, A. Cazares, F. Baganz, R. Wohlgemuth, H.C. Hailes, N. Szita, J. Mol. Catal. B Enym., 77, 1-8.

Investigating the reaction mechanism and organocatalytic synthesis of α,α′-dihydroxy ketones

J.L. Galman, D. Steadman, L.D. Haigh, H.C. Hailes, Org. Biomol. Chem., 10, 2621-8.

An automated microscale platform for evaluation and optimization of oxidative bioconversion processes

J.Z. Baboo, J.L. Galman, G.J. Lye, J.M. Ward, H.C. Hailes, M. Micheletti, Biotechnol. Prog., 28, 392-405.

7.17 C-X Bond Formation: C-C Bond Formation using TDP-Dependent Enzymes

P.A. Dalby, J.M. Ward, H.C. Hailes, Comprehensive Chirality, 7, 372-89.

Convection-Enhanced delivery of neprilysin: A novel amyloid-β- degrading therapeutic strategy

N.U. Barua, J.S. Miners, A.S. Bienemann, M.J. Wyatt, K. Welser, A.B. Tabor, H.C. Hailes, S. Love, S.S. Gill, J. Alzheimer’s Dis., 32, 43-56.

Directed evolution to re-adapt a co-evolved network within an enzyme

J. Strafford, P. Payongsri, E.G. Hibbert, P. Morris, S.S. Batth, D. Steadman, M.E.B. Smith, J.M. Ward, H.C. Hailes, P.A. Dalby, J. Biotechnol., 157, 237-45.

For older references, see here.